Tracing and identifying Primordial germ cells in wild sturgeon stocks

Document Type : Tarvij

Authors

1 Gilan University, Faculty of Basic Sciences

2 international sturgeon research

3 international sturgeon research institute

Abstract

The long-lasting regenerations of sturgeon and their extinction situation depend on the environmental conditions and the nature evolution of these species, which are part of the old ones, and also, the high demand for caviar, which is one of the luxury and expensive products of this species; has led to the reduction of their generation. Different methods have been used for regenerated of these species, among which the usual method is reproduction in breeding environment and release of fry in natural water sources of these species. With the advancement of biological technology and the identification of primordial germ cells, it is possible to isolate cells, track them with molecular markers such as nanos1, vasa and dead end, gene expression or non-molecular markers such as FITC dextran; cultivation and storage of germ stem cells in liquid nitrogen, thawing of cells and use for transplanting to the Recipient animal are presented. Meanwhile, the use of morpholino biotechnology in removing the gene sequence, the main genes involved in the function of Primordial germ cells, as well as the use of gene editing techniques such as CRISPER /CAS9, can provide a suitable way to sterilize the recipient of the transplant fish.

Keywords